{"medium":{"id":"535a","name":"TRYPTICASE SOY BROTH AGAR+BLOOD","complex_medium":"yes","source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535a.pdf"},"solutions":[{"id":1182,"name":"Main sol. 535a","volume":1000,"recipe":[{"recipe No.":1,"solution":"Main sol. 535","solution_id":1181,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"Sheep blood","compound_id":2047,"amount":5,"unit":"%","g_l":50,"optional":"no"}],"steps":[{"step":"To medium 535 add 5% blood after cooling."}],"main solution":"yes"},{"id":1181,"name":"Main sol. 535","volume":1000,"recipe":[{"recipe No.":1,"compound":"Trypticase soy broth","compound_id":99,"attribute":"BBL 11768, Oxoid CM129 or Merck 5459","amount":30,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"Agar","compound_id":3,"amount":20,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"pH 7.3"},{"step":"Autoclave at 121\u00b0C for 15 min."}],"equipment":{"2366":"Autoclave"},"main solution":"no"}]}