{"medium":{"id":52,"name":"CLOSTRIDIUM KLUYVERI MEDIUM","complex_medium":"yes","min_pH":6.8,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium52.pdf"},"solutions":[{"id":67,"name":"Main sol. 52","volume":1023,"recipe":[{"recipe No.":1,"compound":"K-acetate","compound_id":86,"amount":10,"unit":"g","g_l":10,"mmol_l":101.893,"optional":"no"},{"recipe No.":2,"compound":"K2HPO4","compound_id":10,"amount":0.31,"unit":"g","g_l":0.31,"mmol_l":1.77981,"optional":"no"},{"recipe No.":3,"compound":"KH2PO4","compound_id":11,"amount":0.23,"unit":"g","g_l":0.23,"mmol_l":1.69012,"optional":"no"},{"recipe No.":4,"compound":"NH4Cl","compound_id":42,"amount":0.25,"unit":"g","g_l":0.25,"mmol_l":4.67377,"optional":"no"},{"recipe No.":5,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.2,"unit":"g","g_l":0.2,"mmol_l":0.811438,"optional":"no"},{"recipe No.":6,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":7,"solution":"Selenite-tungstate solution","solution_id":777,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":8,"compound":"Yeast extract","compound_id":16,"amount":1,"unit":"g","g_l":1,"optional":"no"},{"recipe No.":9,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0005,"optional":"no"},{"recipe No.":10,"compound":"Ethanol absolute","compound_id":89,"amount":20,"unit":"ml","optional":"no"},{"recipe No.":11,"compound":"Na2CO3","compound_id":32,"amount":1,"unit":"g","g_l":1,"mmol_l":9.43503,"optional":"no"},{"recipe No.":12,"solution":"Seven vitamins solution","solution_id":1038,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":13,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.25,"unit":"g","g_l":0.25,"mmol_l":1.4234,"optional":"no"},{"recipe No.":14,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.25,"unit":"g","g_l":0.25,"mmol_l":1.04088,"optional":"no"},{"recipe No.":15,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve all ingredients (except ethanol, carbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add ethanol, then dispense medium under same gas atmosphere in Hungate-type tubes or serum vials and autoclave. After autoclaving, add carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and vitamins from an anoxic and filter-sterilized stock solution prepared under 100% N2 gas. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of medium to 6.8 - 7.0, if necessary."},{"step":"Note: If reduction of medium is not complete after inoculation, add 10 - 20 mg/l sodium dithionite from a 5% w/v solution freshly prepared under 100% N2 gas and filter sterilized."}],"equipment":{"1052":"Autoclave","1861":"Hungate tubes","2891":"Filter","3945":"Gassing station"},"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":777,"name":"Selenite-tungstate solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":12.5009,"optional":"no"},{"recipe No.":2,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0114062,"optional":"no"},{"recipe No.":3,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.0121267,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":3,"main solution":"no"},{"id":1038,"name":"Seven vitamins solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Vitamin B12","compound_id":18,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.073779,"optional":"no"},{"recipe No.":2,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":80,"unit":"mg","g_l":0.08,"mmol_l":0.583345,"optional":"no"},{"recipe No.":3,"compound":"D-(+)-biotin","compound_id":1359,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":4,"compound":"Nicotinic acid","compound_id":139,"amount":200,"unit":"mg","g_l":0.2,"mmol_l":1.62456,"optional":"no"},{"recipe No.":5,"compound":"Calcium pantothenate","compound_id":518,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.209849,"optional":"no"},{"recipe No.":6,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":300,"unit":"mg","g_l":0.3,"mmol_l":1.45886,"optional":"no"},{"recipe No.":7,"compound":"Thiamine-HCl x 2 H2O","compound_id":77,"amount":200,"unit":"mg","g_l":0.2,"mmol_l":0.535761,"optional":"no"},{"recipe No.":8,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":4,"main solution":"no"}]}