{"medium":{"id":"514g","name":"HALF STRENGTH BACTO MARINE BROTH with 50% Seawater","complex_medium":"yes","min_pH":7.6,"max_pH":7.6,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514g.pdf"},"solutions":[{"id":1142,"name":"Main sol. 514g","volume":1000,"recipe":[{"recipe No.":1,"compound":"Bacto peptone","compound_id":19,"amount":2.5,"unit":"g","g_l":2.5,"optional":"no"},{"recipe No.":2,"compound":"Yeast extract","compound_id":16,"attribute":"Bacto ","amount":0.5,"unit":"g","g_l":0.5,"optional":"no"},{"recipe No.":3,"compound":"Fe(III) citrate","compound_id":45,"amount":0.05,"unit":"g","g_l":0.05,"mmol_l":0.204128,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":9.725,"unit":"g","g_l":9.725,"mmol_l":166.41,"optional":"no"},{"recipe No.":5,"compound":"MgCl2","compound_id":453,"attribute":"anhydrous","amount":2.95,"unit":"g","g_l":2.95,"mmol_l":30.9841,"optional":"no"},{"recipe No.":6,"compound":"Na2SO4","compound_id":110,"amount":1.62,"unit":"g","g_l":1.62,"mmol_l":11.4052,"optional":"no"},{"recipe No.":7,"compound":"CaCl2","compound_id":81,"amount":0.9,"unit":"g","g_l":0.9,"mmol_l":8.10957,"optional":"no"},{"recipe No.":8,"compound":"KCl","compound_id":61,"amount":0.275,"unit":"g","g_l":0.275,"mmol_l":3.6888,"optional":"no"},{"recipe No.":9,"compound":"NaHCO3","compound_id":55,"amount":0.08,"unit":"g","g_l":0.08,"mmol_l":0.952302,"optional":"no"},{"recipe No.":10,"compound":"KBr","compound_id":174,"amount":0.04,"unit":"g","g_l":0.04,"mmol_l":0.336134,"optional":"no"},{"recipe No.":11,"compound":"SrCl2","compound_id":527,"amount":17,"unit":"mg","g_l":0.017,"mmol_l":0.107242,"optional":"no"},{"recipe No.":12,"compound":"H3BO3","compound_id":37,"amount":11,"unit":"mg","g_l":0.011,"mmol_l":0.177878,"optional":"no"},{"recipe No.":13,"compound":"Na-silicate","compound_id":524,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.016385,"optional":"no"},{"recipe No.":14,"compound":"NaF","compound_id":177,"amount":1.2,"unit":"mg","g_l":0.0012,"mmol_l":0.0285795,"optional":"no"},{"recipe No.":15,"compound":"(NH4)NO3","compound_id":528,"amount":0.8,"unit":"mg","g_l":0.0008,"mmol_l":0.00999458,"optional":"no"},{"recipe No.":16,"compound":"Na2HPO4","compound_id":233,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.0281771,"optional":"no"},{"recipe No.":17,"compound":"Sea water","compound_id":286,"amount":500,"unit":"ml","optional":"no"},{"recipe No.":19,"compound":"Distilled water","compound_id":4,"amount":500,"unit":"ml","optional":"no"},{"recipe No.":20,"compound":"Agar","compound_id":3,"condition":"for solid medium","amount":15,"unit":"g/l","g_l":15,"mmol_l":44.5991,"optional":"no"}],"steps":[{"step":"Final pH should be 7.6 \u00b1 0.2 at 25\u00b0C. If using Difco Bacto Marine Broth use 18.7 g/l to 1 litre water."},{"step":"The medium may be solidified by adding 15 g/l agar."}],"main solution":"yes"}]}