{"medium":{"id":"514c","name":"REACTIVATION WITH LIQUID MEDIUM 514","complex_medium":"yes","min_pH":7.6,"max_pH":7.6,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514c.pdf"},"solutions":[{"id":1137,"name":"Main sol. 514c","volume":1000,"recipe":[{"recipe No.":1,"solution":"514. BACTO MARINE BROTH (DIFCO 2216)","solution_id":1138,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 514. Subsequent subculturing may be carried out in liquid medium or on agar plates."}],"main solution":"yes"},{"id":1138,"name":"514. BACTO MARINE BROTH (DIFCO 2216)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Bacto peptone","compound_id":19,"amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":2,"compound":"Yeast extract","compound_id":16,"attribute":"Bacto ","amount":1,"unit":"g","g_l":1,"optional":"no"},{"recipe No.":3,"compound":"Fe(III) citrate","compound_id":45,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.408257,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":19.45,"unit":"g","g_l":19.45,"mmol_l":332.82,"optional":"no"},{"recipe No.":5,"compound":"MgCl2","compound_id":453,"attribute":"dried","amount":5.9,"unit":"g","g_l":5.9,"mmol_l":61.9683,"optional":"no"},{"recipe No.":6,"compound":"Na2SO4","compound_id":110,"amount":3.24,"unit":"g","g_l":3.24,"mmol_l":22.8105,"optional":"no"},{"recipe No.":7,"compound":"CaCl2","compound_id":81,"amount":1.8,"unit":"g","g_l":1.8,"mmol_l":16.2191,"optional":"no"},{"recipe No.":8,"compound":"KCl","compound_id":61,"amount":0.55,"unit":"g","g_l":0.55,"mmol_l":7.3776,"optional":"no"},{"recipe No.":9,"compound":"Na2CO3","compound_id":32,"amount":0.16,"unit":"g","g_l":0.16,"mmol_l":1.5096,"optional":"no"},{"recipe No.":10,"compound":"KBr","compound_id":174,"amount":0.08,"unit":"g","g_l":0.08,"mmol_l":0.672269,"optional":"no"},{"recipe No.":11,"compound":"SrCl2","compound_id":527,"amount":34,"unit":"mg","g_l":0.034,"mmol_l":0.214484,"optional":"no"},{"recipe No.":12,"compound":"H3BO3","compound_id":37,"amount":22,"unit":"mg","g_l":0.022,"mmol_l":0.355757,"optional":"no"},{"recipe No.":13,"compound":"Na-silicate","compound_id":524,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.03277,"optional":"no"},{"recipe No.":14,"compound":"NaF","compound_id":177,"amount":2.4,"unit":"mg","g_l":0.0024,"mmol_l":0.0571589,"optional":"no"},{"recipe No.":15,"compound":"(NH4)NO3","compound_id":528,"amount":1.6,"unit":"mg","g_l":0.0016,"mmol_l":0.0199892,"optional":"no"},{"recipe No.":16,"compound":"Na2HPO4","compound_id":233,"amount":8,"unit":"mg","g_l":0.008,"mmol_l":0.0563543,"optional":"no"},{"recipe No.":17,"compound":"Agar","compound_id":3,"amount":18,"unit":"g","g_l":18,"mmol_l":53.5189,"optional":"no"},{"recipe No.":18,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Final pH should be 7.6 \u00b1 0.2 at 25\u00b0C. If using the complete medium from Difco add 37.4 g to 1 litre water."}],"main solution":"no"}]}