{"medium":{"id":465,"name":"MINERAL MEDIUM PH 7.25","complex_medium":"yes","min_pH":7.2,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465.pdf"},"solutions":[{"id":945,"name":"Main sol. 465","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na2HPO4 x 2 H2O","compound_id":181,"amount":3.5,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"KH2PO4","compound_id":11,"amount":1,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"(NH4)2SO4","compound_id":27,"amount":0.5,"unit":"g","optional":"no"},{"recipe No.":4,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.1,"unit":"g","optional":"no"},{"recipe No.":5,"compound":"Ca(NO3)2 x 4 H2O","compound_id":213,"amount":0.05,"unit":"g","optional":"no"},{"recipe No.":6,"solution":"Trace element solution SL-4","solution_id":20,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"pH 7.25"},{"step":"Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source."}],"main solution":"yes"},{"id":1,"name":"Main sol. 1","volume":1000,"recipe":[{"recipe No.":1,"compound":"Peptone","compound_id":1,"amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":2,"compound":"Meat extract","compound_id":2,"amount":3,"unit":"g","g_l":3,"optional":"no"},{"recipe No.":3,"compound":"Agar","compound_id":3,"condition":"for solid medium","amount":15,"unit":"g","g_l":15,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.0."},{"step":"For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation."}],"main solution":"no"},{"id":477,"name":"Main sol. 220","volume":1000,"recipe":[{"recipe No.":1,"compound":"Casein peptone","compound_id":21,"amount":15,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"Soy peptone","compound_id":75,"amount":5,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"NaCl","compound_id":43,"amount":5,"unit":"g","optional":"no"},{"recipe No.":4,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","optional":"no"},{"recipe No.":5,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131)."}],"main solution":"no"},{"id":1181,"name":"Main sol. 535","volume":1000,"recipe":[{"recipe No.":1,"compound":"Trypticase soy broth","compound_id":99,"attribute":"BBL 11768, Oxoid CM129 or Merck 5459","amount":30,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"Agar","compound_id":3,"amount":20,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"pH 7.3"},{"step":"Autoclave at 121\u00b0C for 15 min."}],"equipment":{"2366":"Autoclave"},"main solution":"no"},{"id":1685,"name":"Main sol. 830","volume":1000,"recipe":[{"recipe No.":1,"compound":"Yeast extract","compound_id":16,"amount":0.5,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"Proteose peptone","compound_id":208,"attribute":"Difco no. 3","amount":0.5,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"Casamino acids","compound_id":101,"amount":0.5,"unit":"g","optional":"no"},{"recipe No.":4,"compound":"Glucose","compound_id":5,"amount":0.5,"unit":"g","optional":"no"},{"recipe No.":5,"compound":"Starch","compound_id":199,"attribute":"soluble","amount":0.5,"unit":"g","optional":"no"},{"recipe No.":6,"compound":"Na-pyruvate","compound_id":113,"amount":0.3,"unit":"g","optional":"no"},{"recipe No.":7,"compound":"K2HPO4","compound_id":10,"amount":0.3,"unit":"g","optional":"no"},{"recipe No.":8,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.05,"unit":"g","optional":"no"},{"recipe No.":9,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121\u00b0C."}],"equipment":{"2128":"Autoclave"},"main solution":"no"},{"id":20,"name":"Trace element solution SL-4","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na2-EDTA","compound_id":34,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":1.48718,"optional":"no"},{"recipe No.":2,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":0.2,"unit":"g","g_l":0.2,"mmol_l":0.719386,"optional":"no"},{"recipe No.":3,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.347752,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.151588,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":0.3,"unit":"g","g_l":0.3,"mmol_l":4.85123,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":0.2,"unit":"g","g_l":0.2,"mmol_l":1.54037,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0586569,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.154322,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.123993,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds."}],"Sol. No.":15,"main solution":"no"}]}