{"medium":{"id":"429d","name":"FRANCISELLA HALIOTICIDA MEDIUM","complex_medium":"yes","source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429d.pdf"},"solutions":[{"id":872,"name":"Main sol. 429d","volume":1000,"recipe":[{"recipe No.":1,"compound":"Columbia agar base","compound_id":2110,"attribute":"dissolved in 70 % artificial seawater","amount":1000,"unit":"ml","g_l":700,"optional":"no"},{"recipe No.":2,"compound":"Agar","compound_id":3,"amount":2,"unit":"g/l","g_l":2,"mmol_l":5.94654,"optional":"no"},{"recipe No.":3,"compound":"Horse blood","compound_id":724,"amount":4,"unit":"%","g_l":40,"optional":"no"},{"recipe No.":5,"compound":"Glucose","compound_id":5,"amount":1,"unit":"%","g_l":10,"mmol_l":55.5062,"optional":"no"},{"recipe No.":6,"compound":"FeCl3","compound_id":403,"amount":2,"unit":"mM","g_l":0.3244,"mmol_l":2,"optional":"no"},{"recipe No.":7,"compound":"Cysteine","compound_id":492,"amount":0.1,"unit":"%","g_l":1,"mmol_l":8.25355,"optional":"no"}],"steps":[{"step":"Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50\u00b0C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215."}],"equipment":{"2090":"Autoclave"},"main solution":"yes"},{"id":477,"name":"Main sol. 220","volume":1000,"recipe":[{"recipe No.":1,"compound":"Casein peptone","compound_id":21,"amount":15,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"Soy peptone","compound_id":75,"amount":5,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"NaCl","compound_id":43,"amount":5,"unit":"g","optional":"no"},{"recipe No.":4,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","optional":"no"},{"recipe No.":5,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131)."}],"main solution":"no"},{"id":469,"name":"Main sol. 215","volume":1000,"recipe":[{"recipe No.":1,"compound":"Brain heart infusion","compound_id":186,"attribute":"Difco","amount":37,"unit":"g","g_l":37,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}