{"medium":{"id":"429c","name":"COLUMBIA-GLUCOSE-CYSTEINE-AGAR","complex_medium":"yes","source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429c.pdf"},"solutions":[{"id":871,"name":"Main sol. 429c","volume":1000,"recipe":[{"recipe No.":1,"compound":"Columbia agar base","compound_id":2110,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"Horse blood","compound_id":724,"amount":4,"unit":"%","g_l":40,"optional":"no"},{"recipe No.":4,"compound":"Glucose","compound_id":5,"amount":1,"unit":"%","g_l":10,"mmol_l":55.5062,"optional":"no"},{"recipe No.":5,"compound":"Cysteine","compound_id":492,"amount":0.1,"unit":"%","g_l":1,"mmol_l":8.25355,"optional":"no"}],"steps":[{"step":"Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50\u00b0C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215."}],"equipment":{"1700":"Autoclave"},"main solution":"yes"},{"id":477,"name":"Main sol. 220","volume":1000,"recipe":[{"recipe No.":1,"compound":"Casein peptone","compound_id":21,"amount":15,"unit":"g","optional":"no"},{"recipe No.":2,"compound":"Soy peptone","compound_id":75,"amount":5,"unit":"g","optional":"no"},{"recipe No.":3,"compound":"NaCl","compound_id":43,"amount":5,"unit":"g","optional":"no"},{"recipe No.":4,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","optional":"no"},{"recipe No.":5,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131)."}],"main solution":"no"},{"id":469,"name":"Main sol. 215","volume":1000,"recipe":[{"recipe No.":1,"compound":"Brain heart infusion","compound_id":186,"attribute":"Difco","amount":37,"unit":"g","g_l":37,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}