{"medium":{"id":411,"name":"CLOSTRIDIUM ACETOBUTYLICUM MEDIUM","complex_medium":"yes","min_pH":6.8,"max_pH":6.8,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium411.pdf"},"solutions":[{"id":840,"name":"Main sol. 411","volume":1000,"recipe":[{"recipe No.":1,"compound":"Potato","compound_id":1606,"condition":"","attribute":"fresh, washed, peeled and sliced","amount":200,"unit":"g","g_l":200,"optional":"no"},{"recipe No.":2,"compound":"CaCO3","compound_id":13,"amount":2,"unit":"g","g_l":2,"mmol_l":19.982,"optional":"no"},{"recipe No.":3,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0005,"optional":"no"},{"recipe No.":4,"compound":"D-Glucose","compound_id":211,"amount":6,"unit":"g","g_l":6,"mmol_l":33.3037,"optional":"no"},{"recipe No.":5,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.8468,"optional":"no"},{"recipe No.":6,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Mix potatoes and CaCO3 then autoclave 30 min at 121\u00b0C. Filter through cheese cloth, add glucose and cysteine, distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121\u00b0C for 20 min. Adjust pH of complete medium to 6.8."},{"step":"Note: For the reactivation of freeze-dried strains it is recommended to use the following simple milk medium:"}],"equipment":{"359":"Autoclave","2442":"Hungate tubes","3483":"Filter","4303":"Gassing station"},"main solution":"yes"},{"id":841,"name":"Milk Medium","volume":100,"recipe":[{"recipe No.":1,"compound":"Milk","compound_id":467,"attribute":"fresh","amount":100,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.1,"unit":"ml","g_l":0.0001,"optional":"no"}],"steps":[{"step":"Adjust pH to 7.1. Tube and autoclave under 100% N2 gas atmosphere for 12 min at 121\u00b0C."}],"equipment":{"1672":"Autoclave"},"main solution":"no"}]}