{"medium":{"id":318,"name":"METHANOSARCINA THERMOPHILA (BCYT) MEDIUM","complex_medium":"yes","min_pH":6.8,"max_pH":6.8,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318.pdf"},"solutions":[{"id":588,"name":"Main sol. 318","volume":1021,"recipe":[{"recipe No.":1,"compound":"KH2PO4","compound_id":11,"amount":0.3,"unit":"g","g_l":0.29383,"mmol_l":2.15916,"optional":"no"},{"recipe No.":2,"compound":"NaCl","compound_id":43,"amount":0.6,"unit":"g","g_l":0.587659,"mmol_l":10.0558,"optional":"no"},{"recipe No.":3,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.1,"unit":"g","g_l":0.0979432,"mmol_l":0.48176,"optional":"no"},{"recipe No.":4,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.08,"unit":"g","g_l":0.0783546,"mmol_l":0.53297,"optional":"no"},{"recipe No.":5,"solution":"Trace element solution","solution_id":589,"attribute":"","amount":10,"unit":"ml","optional":"no"},{"recipe No.":6,"compound":"NH4Cl","compound_id":42,"amount":1,"unit":"g","g_l":0.979432,"mmol_l":18.3106,"optional":"no"},{"recipe No.":7,"compound":"Yeast extract","compound_id":16,"attribute":"OXOID","amount":0.5,"unit":"g","g_l":0.489716,"optional":"no"},{"recipe No.":8,"compound":"Trypticase peptone","compound_id":124,"attribute":"BD BBL","amount":0.5,"unit":"g","g_l":0.489716,"optional":"no"},{"recipe No.":9,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000489716,"mmol_l":0.00194974,"optional":"no"},{"recipe No.":10,"compound":"KHCO3","compound_id":370,"amount":2,"unit":"g","g_l":1.95886,"mmol_l":19.5661,"optional":"no"},{"recipe No.":11,"compound":"Methanol","compound_id":226,"attribute":"50% v/v","amount":10,"unit":"ml","g_l":7.7571,"mmol_l":242.092,"optional":"no"},{"recipe No.":12,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":13,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.3,"unit":"g","g_l":0.29383,"mmol_l":1.67295,"optional":"no"},{"recipe No.":14,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":0.29383,"mmol_l":1.22336,"optional":"no"},{"recipe No.":15,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except bicarbonate, vitamins, methanol, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins (sterilized by filtration), methanol (50% v/v solution), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8, if necessary."}],"equipment":{"894":"Filter","1155":"Hungate tubes","2939":"Autoclave","4263":"Gassing station"},"main solution":"yes"},{"id":589,"name":"Trace element solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"attribute":"NTA","amount":12.8,"unit":"g","g_l":12.8,"mmol_l":66.9666,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1,"unit":"g","g_l":1,"mmol_l":5.02988,"optional":"no"},{"recipe No.":3,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":4,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.231055,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.680203,"optional":"no"},{"recipe No.":6,"compound":"ZnCl2","compound_id":64,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.733676,"optional":"no"},{"recipe No.":7,"compound":"CuCl2","compound_id":261,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.148754,"optional":"no"},{"recipe No.":8,"compound":"H3BO3","compound_id":37,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.161708,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.123993,"optional":"no"},{"recipe No.":10,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.771611,"optional":"no"},{"recipe No.":11,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","g_l":1,"mmol_l":17.1116,"optional":"no"},{"recipe No.":12,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.114062,"optional":"no"},{"recipe No.":13,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":0.04,"unit":"g","g_l":0.04,"mmol_l":0.121267,"optional":"no"},{"recipe No.":14,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml."}],"Sol. No.":24,"main solution":"no"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}