{"medium":{"id":"311b","name":"SPOROMUSA MEDIUM (MARINE)","complex_medium":"yes","min_pH":7.5,"max_pH":7.7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311b.pdf"},"solutions":[{"id":578,"name":"Main sol. 311b","volume":1005,"recipe":[{"recipe No.":1,"compound":"NH4Cl","compound_id":42,"amount":0.5,"unit":"g","g_l":0.497512,"mmol_l":9.30104,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.5,"unit":"g","g_l":0.497512,"mmol_l":2.0185,"optional":"no"},{"recipe No.":3,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.25,"unit":"g","g_l":0.248756,"mmol_l":1.69205,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":25,"unit":"g","g_l":24.8756,"mmol_l":425.661,"optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"attribute":"0.1% w/v in 0.1 N H2SO4","amount":2,"unit":"ml","g_l":0.00199005,"mmol_l":0.00715807,"optional":"no"},{"recipe No.":6,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":7,"solution":"Selenite-tungstate solution","solution_id":777,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":8,"compound":"Yeast extract","compound_id":16,"amount":2,"unit":"g","g_l":1.99005,"optional":"no"},{"recipe No.":9,"compound":"Casitone","compound_id":84,"amount":2,"unit":"g","g_l":1.99005,"optional":"no"},{"recipe No.":10,"compound":"Betaine x H2O","compound_id":212,"amount":6.7,"unit":"g","g_l":6.66667,"mmol_l":56.909,"optional":"no"},{"recipe No.":11,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000497512,"mmol_l":0.00198078,"optional":"no"},{"recipe No.":12,"compound":"K2HPO4","compound_id":10,"amount":0.35,"unit":"g","g_l":0.348259,"mmol_l":1.99946,"optional":"no"},{"recipe No.":13,"compound":"KH2PO4","compound_id":11,"amount":0.23,"unit":"g","g_l":0.228856,"mmol_l":1.68171,"optional":"no"},{"recipe No.":14,"compound":"Na2CO3","compound_id":32,"amount":1.5,"unit":"g","g_l":1.49254,"mmol_l":14.0821,"optional":"no"},{"recipe No.":15,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":16,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.3,"unit":"g","g_l":0.298507,"mmol_l":1.69958,"optional":"no"},{"recipe No.":17,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":0.298507,"mmol_l":1.24284,"optional":"no"},{"recipe No.":18,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients (except phosphates, carbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.5 - 7.7."}],"equipment":{"1279":"Autoclave","2082":"Hungate tubes","3103":"Filter","4258":"Gassing station"},"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":777,"name":"Selenite-tungstate solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":12.5009,"optional":"no"},{"recipe No.":2,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0114062,"optional":"no"},{"recipe No.":3,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.0121267,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":3,"main solution":"no"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}