{"medium":{"id":31,"name":"ALKALINE NUTRIENT AGAR","complex_medium":"yes","min_pH":9.7,"max_pH":9.7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium31.pdf"},"solutions":[{"id":5552,"name":"Main sol. 31","volume":1000,"recipe":[{"recipe No.":1,"compound":"Peptone","compound_id":1,"amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":2,"compound":"Meat extract","compound_id":2,"amount":3,"unit":"g","g_l":3,"optional":"no"},{"recipe No.":3,"compound":"Agar","compound_id":3,"condition":"if necessary","amount":15,"unit":"g","g_l":15,"mmol_l":44.5991,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":900,"unit":"ml","optional":"no"},{"recipe No.":5,"solution":"Na-sesquicarbonate solution","solution_id":46,"amount":100,"unit":"ml","optional":"no"}],"steps":[{"step":"For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation."},{"recipe No.":5,"step":"After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml)  to achieve a pH of 9.7"}],"equipment":{"4014":"Autoclave"},"main solution":"yes"},{"id":46,"name":"Na-sesquicarbonate solution","volume":100,"recipe":[{"recipe No.":1,"compound":"NaHCO3","compound_id":55,"amount":4.2,"unit":"g","g_l":42,"mmol_l":499.958,"optional":"no"},{"recipe No.":2,"compound":"Na2CO3 anhydrous","compound_id":69,"amount":5.3,"unit":"g","g_l":53,"mmol_l":500.057,"optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":100,"unit":"ml","optional":"no"}],"main solution":"no"}]}