{"medium":{"id":209,"name":"EUBACTERIUM LENTUM MEDIUM","complex_medium":"yes","min_pH":7,"max_pH":7,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium209.pdf"},"solutions":[{"id":441,"name":"Main sol. 209","volume":1000,"recipe":[{"recipe No.":1,"solution":"Main sol. 78","solution_id":123,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"Arginine","compound_id":326,"amount":0.5,"unit":"%(w/v)","g_l":5,"mmol_l":28.7026,"optional":"no"}],"steps":[{"step":"To medium 78 add 0.5% (w/v) arginine."}],"main solution":"yes"},{"id":123,"name":"Main sol. 78","volume":1025,"recipe":[{"recipe No.":1,"compound":"Ground beef","compound_id":128,"attribute":"fat free","amount":500,"unit":"g","g_l":487.805,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":3,"compound":"NaOH","compound_id":256,"attribute":"1 N","amount":25,"unit":"ml","optional":"no"},{"recipe No.":4,"compound":"Casitone","compound_id":84,"amount":30,"unit":"g","g_l":29.2683,"optional":"no"},{"recipe No.":5,"compound":"Yeast extract","compound_id":16,"amount":5,"unit":"g","g_l":4.87805,"optional":"no"},{"recipe No.":6,"compound":"K2HPO4","compound_id":10,"amount":5,"unit":"g","g_l":4.87805,"mmol_l":28.0064,"optional":"no"},{"recipe No.":7,"compound":"Resazurin","compound_id":25,"amount":1,"unit":"mg","g_l":0.00097561,"mmol_l":0.00425677,"optional":"no"},{"recipe No.":8,"compound":"L-Cysteine HCl","compound_id":1157,"condition":"","amount":0.5,"unit":"g","g_l":0.487805,"mmol_l":3.09482,"optional":"no"},{"recipe No.":9,"compound":"Agar","compound_id":3,"condition":"for solid medium","amount":15,"unit":"g","g_l":14.6341,"optional":"no"},{"recipe No.":10,"solution":"Haemin solution","solution_id":124,"amount":10,"unit":"ml","optional":"yes"},{"recipe No.":11,"solution":"Vitamin K1 solution","solution_id":125,"condition":"alternative","amount":0.2,"unit":"ml","optional":"yes"},{"recipe No.":12,"solution":"Vitamin K3 solution","solution_id":5540,"condition":"alternative","amount":10,"unit":"ml","optional":"yes"}],"steps":[{"recipe No.":8,"step":"To make medium anoxic bring it to a boil, cool under 100% N2 gas atmosphere, add 0.5 g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under 100% N2 gas atmosphere by filling 7 ml medium into anoxic Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121\u00b0C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium."},{"recipe No.":10,"step":"In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving:"},{"recipe No.":4,"step":"Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix\r\nmeat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature,\r\nskim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate\r\nadd water to a final volume of 1000 ml, and then add:"}],"equipment":{"429":"Hungate tubes","1480":"Filter","3487":"Autoclave","3958":"Gassing station"},"main solution":"no"},{"id":124,"name":"Haemin solution","volume":100,"recipe":[{"recipe No.":1,"compound":"Haemin","compound_id":149,"amount":50,"unit":"mg","g_l":0.5,"mmol_l":0.766989,"optional":"no"},{"recipe No.":2,"compound":"NaOH","compound_id":256,"attribute":"1 N","amount":1,"unit":"ml","optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":100,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated."}],"equipment":{"1875":"Filter"},"Sol. No.":9,"main solution":"no"},{"id":5540,"name":"Vitamin K3 solution","volume":100,"recipe":[{"recipe No.":1,"compound":"Vitamin K3","compound_id":2086,"amount":5,"unit":"mg","g_l":0.05,"optional":"no"},{"recipe No.":2,"compound":"Ethanol","compound_id":201,"attribute":"95%","amount":1,"unit":"ml","g_l":9.5,"mmol_l":206.208,"optional":"no"},{"recipe No.":3,"compound":"Water","compound_id":754,"amount":100,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle."}],"main solution":"no"},{"id":125,"name":"Vitamin K1 solution","volume":20,"recipe":[{"recipe No.":1,"compound":"Vitamin K1","compound_id":150,"amount":0.1,"unit":"ml","optional":"no"},{"recipe No.":2,"compound":"Ethanol","compound_id":201,"attribute":"95 %","amount":20,"unit":"ml","g_l":950,"mmol_l":20620.8,"optional":"no"}],"steps":[{"step":"Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle."}],"equipment":{"277":"Filter"},"Sol. No.":20,"main solution":"no"}]}