{"medium":{"id":1884,"name":"Linsmaier & Skoog LS Plant Cell Medium","complex_medium":"yes","min_pH":6,"max_pH":6,"source":"DSMZ","description":"Medium used for cultivation of \r\nPC 44, PC 273, PC 655 and PC 1017 plant cell lines.\r\nModified after LINSMAIER, E.M. and F. SKOOG: Physiol. Plant., 18, 100-127, (1965)"},"solutions":[{"id":6606,"name":"Main Solution 1884","volume":1000,"recipe":[{"recipe No.":1,"compound":"Linsmaier & Skoog Med. incl. vitamins","compound_id":2431,"attribute":"Duchefa L0230","amount":4.4,"unit":"g","g_l":4.4,"optional":"no"},{"recipe No.":2,"solution":"2,4-Dichlorophenoxyacetic Acid Stock Solution","solution_id":6592,"amount":0.22,"unit":"ml","optional":"no"},{"recipe No.":3,"solution":"1-Naphtylacetic Acid Stock Solution","solution_id":6607,"amount":0.186,"unit":"ml","optional":"no"},{"recipe No.":4,"compound":"Sucrose","compound_id":108,"attribute":"Duchefa S0809","amount":30,"unit":"g","g_l":30,"mmol_l":87.6424,"optional":"no"},{"recipe No.":8,"compound":"Plant Agar","compound_id":2441,"attribute":"Duchefa P1001","amount":9,"unit":"g","g_l":9,"optional":"no"}],"steps":[{"recipe No.":5,"step":"Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water."},{"recipe No.":6,"step":"Adjust pH to 6.0."},{"recipe No.":7,"step":"For solid medium add Plant Agar as followed:"},{"step":"Autoclave at 121 \u00b0C for 20 min."}],"equipment":{"5454":"Autoclave"},"main solution":"yes"},{"id":6592,"name":"2,4-Dichlorophenoxyacetic Acid Stock Solution","volume":100,"recipe":[{"recipe No.":1,"compound":"2,4-Dichlorophenoxyacetic Acid","compound_id":2102,"attribute":"Duchefa D0911","amount":100,"unit":"mg","g_l":1,"optional":"no"},{"recipe No.":2,"compound":"Ethanol absolute","compound_id":89,"amount":100,"unit":"ml","optional":"no"}],"steps":[{"step":"Store in a glass bottle with a ground glass stopper at 4 \u00b0C."}],"main solution":"no"},{"id":6607,"name":"1-Naphtylacetic Acid Stock Solution","volume":100,"recipe":[{"recipe No.":1,"compound":"1-Naphtylacetic Acid","compound_id":2436,"attribute":"Duchefa N0903","amount":100,"unit":"mg","g_l":1,"optional":"no"},{"recipe No.":2,"compound":"Ethanol absolute","compound_id":89,"amount":100,"unit":"ml","optional":"no"}],"steps":[{"step":"To prepare a 100 mg/ml stock solution, dissolve 100 mg of 1-Naphthylacetic Acid (NAA) in a few millilitres of 1 M NaOH, then fill up to 100 ml with absolute ethanol."},{"step":"Store in a glass bottle with a ground glass stopper at 4 \u00b0C."}],"main solution":"no"}]}