{"medium":{"id":1669,"name":"SCHAEDLER BROTH (Roth; 5772)","complex_medium":"yes","min_pH":7.6,"max_pH":7.6,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1669.pdf"},"solutions":[{"id":3464,"name":"Main sol. 1669","volume":1000,"recipe":[{"recipe No.":1,"compound":"Casein peptone","compound_id":21,"amount":5.66,"unit":"g","g_l":5.66,"optional":"no"},{"recipe No.":2,"compound":"Soy peptone","compound_id":75,"amount":1,"unit":"g","g_l":1,"optional":"no"},{"recipe No.":3,"compound":"Yeast extract","compound_id":16,"amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":4,"compound":"Peptone mixture","compound_id":156,"amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":5,"compound":"Glucose","compound_id":5,"amount":5.83,"unit":"g","g_l":5.83,"mmol_l":32.3601,"optional":"no"},{"recipe No.":6,"compound":"K2HPO4","compound_id":10,"amount":0.83,"unit":"g","g_l":0.83,"mmol_l":4.7653,"optional":"no"},{"recipe No.":7,"compound":"NaCl","compound_id":43,"amount":1.66,"unit":"g","g_l":1.66,"mmol_l":28.4052,"optional":"no"},{"recipe No.":8,"compound":"Tris","compound_id":73,"attribute":"hydroxymethyl aminomethane","amount":3,"unit":"g","g_l":3,"mmol_l":24.7647,"optional":"no"},{"recipe No.":9,"compound":"Resazurin","compound_id":25,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00436319,"optional":"no"},{"recipe No.":10,"compound":"Hemin","compound_id":704,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0153398,"optional":"no"},{"recipe No.":11,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.4,"unit":"g","g_l":0.4,"mmol_l":2.27744,"optional":"no"},{"recipe No.":12,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Boil medium under CO2 until the resazurin indicator changes from pink to colourless. Cool down under CO2 and adjust pH to 7.6. Switch to N2 to prevent further lowering of the pH. Fill up into Hungate tubes under N2 and autoclave."}],"equipment":{"517":"Autoclave","1346":"Hungate tubes","3100":"Gassing station"},"main solution":"yes"}]}