{"medium":{"id":1629,"name":"MINIMAL MEDIA CONTAINING METHANOL","complex_medium":"no","min_pH":7.1,"max_pH":7.1,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1629.pdf"},"solutions":[{"id":3381,"name":"Main sol. 1629","volume":1000,"recipe":[{"recipe No.":1,"compound":"NH4Cl","compound_id":42,"amount":1.62,"unit":"g","g_l":1.62,"mmol_l":30.286,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.2,"unit":"g","g_l":0.2,"mmol_l":0.811438,"optional":"no"},{"recipe No.":3,"compound":"K2HPO4","compound_id":10,"amount":2.4,"unit":"g","g_l":2.4,"mmol_l":13.7792,"optional":"no"},{"recipe No.":4,"compound":"NaH2PO4 x 2 H2O","compound_id":678,"amount":1.1,"unit":"g","g_l":1.1,"mmol_l":9.16842,"optional":"no"},{"recipe No.":5,"compound":"Na2-EDTA","compound_id":34,"amount":15,"unit":"mg","g_l":0.015,"mmol_l":0.0446155,"optional":"no"},{"recipe No.":6,"compound":"FeSO4 x 6 H2O","compound_id":1217,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0197488,"optional":"no"},{"recipe No.":7,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":4.5,"unit":"mg","g_l":0.0045,"mmol_l":0.0156489,"optional":"no"},{"recipe No.":8,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0231055,"optional":"no"},{"recipe No.":9,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":0.64,"unit":"mg","g_l":0.00064,"mmol_l":0.00323387,"optional":"no"},{"recipe No.":10,"compound":"H3BO3","compound_id":37,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.0161708,"optional":"no"},{"recipe No.":11,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.4,"unit":"mg","g_l":0.0004,"mmol_l":0.00165323,"optional":"no"},{"recipe No.":12,"compound":"CuSO4 x 2 H2O","compound_id":719,"amount":0.3,"unit":"mg","g_l":0.0003,"mmol_l":0.00187958,"optional":"no"},{"recipe No.":13,"compound":"CaCl2 x 6 H2O","compound_id":173,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0136939,"optional":"no"},{"recipe No.":14,"compound":"Methanol","compound_id":226,"amount":5,"unit":"ml","g_l":3.96,"mmol_l":123.588,"optional":"no"},{"recipe No.":15,"compound":"Agar","compound_id":3,"amount":15,"unit":"g","g_l":15,"mmol_l":44.5991,"optional":"no"},{"recipe No.":16,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Prepare the medium without methanol. Adjust pH to 7.1."},{"step":"Autoclave and after cooling to 50\u00b0C add the methanol from a filter-sterilized stock solution."}],"equipment":{"2":"Autoclave","3130":"Filter"},"main solution":"yes"}]}