{"medium":{"id":1584,"name":"DESULFUROMONAS CARBONIS MEDIUM","complex_medium":"no","min_pH":7,"max_pH":7.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1584.pdf"},"solutions":[{"id":3274,"name":"Main sol. 1584","volume":1015,"recipe":[{"recipe No.":1,"compound":"NaCl","compound_id":43,"amount":8.8,"unit":"g","g_l":8.66995,"mmol_l":148.356,"optional":"no"},{"recipe No.":2,"compound":"KCl","compound_id":61,"amount":0.34,"unit":"g","g_l":0.334975,"mmol_l":4.4933,"optional":"no"},{"recipe No.":3,"compound":"NH4Cl","compound_id":42,"amount":0.25,"unit":"g","g_l":0.246305,"mmol_l":4.6047,"optional":"no"},{"recipe No.":4,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":4.84,"unit":"g","g_l":4.76847,"mmol_l":23.455,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.15,"unit":"g","g_l":0.147783,"mmol_l":1.00523,"optional":"no"},{"recipe No.":6,"compound":"K2HPO4","compound_id":10,"amount":0.15,"unit":"g","g_l":0.147783,"mmol_l":0.848471,"optional":"no"},{"recipe No.":7,"compound":"Fe(NH4)2(SO4)2","compound_id":106,"attribute":"0.1% w/v","amount":3,"unit":"ml","g_l":0.00295567,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"attribute":"0.1% w/v","amount":9,"unit":"ml","g_l":0.00886699,"mmol_l":0.0684187,"optional":"no"},{"recipe No.":9,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":10,"solution":"Selenite-tungstate solution","solution_id":777,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":11,"compound":"Na2CO3","compound_id":32,"amount":1.5,"unit":"g","g_l":1.47783,"mmol_l":13.9434,"optional":"no"},{"recipe No.":12,"compound":"Na2-fumarate","compound_id":276,"amount":8,"unit":"g","g_l":7.88177,"mmol_l":49.25,"optional":"no"},{"recipe No.":13,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":14,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients (except carbonate, fumarate and vitamins) and sparge medium with 80% N2 and 20% CO2 gas mixture to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding fumarate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of fumarate and vitamins are sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2, if necessary."}],"equipment":{"1955":"Autoclave","2748":"Hungate tubes","3775":"Filter","4205":"Gassing station"},"main solution":"yes"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":777,"name":"Selenite-tungstate solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":12.5009,"optional":"no"},{"recipe No.":2,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":3,"unit":"mg","g_l":0.003,"mmol_l":0.0114062,"optional":"no"},{"recipe No.":3,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":4,"unit":"mg","g_l":0.004,"mmol_l":0.0121267,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":3,"main solution":"no"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}