{"medium":{"id":1486,"name":"GBS salts modified for Thermocrinis jamiesonii","complex_medium":"no","min_pH":7.2,"max_pH":7.3,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1486.pdf"},"solutions":[{"id":3047,"name":"Main sol. 1486","volume":1000,"recipe":[{"recipe No.":1,"compound":"NaH2PO4","compound_id":229,"amount":1.38,"unit":"g","g_l":1.38,"mmol_l":11.5022,"optional":"no"},{"recipe No.":2,"compound":"NaCl","compound_id":43,"amount":3,"unit":"g","g_l":3,"mmol_l":51.3347,"optional":"no"},{"recipe No.":3,"compound":"NH4Cl","compound_id":42,"amount":0.107,"unit":"g","g_l":0.107,"mmol_l":2.00037,"optional":"no"},{"recipe No.":4,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.015,"unit":"g","g_l":0.015,"mmol_l":0.10203,"optional":"no"},{"recipe No.":5,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.123,"unit":"g","g_l":0.123,"mmol_l":0.499034,"optional":"no"},{"recipe No.":6,"compound":"KCl","compound_id":61,"amount":0.15,"unit":"g","g_l":0.15,"mmol_l":2.01207,"optional":"no"},{"recipe No.":7,"compound":"Na2SO4","compound_id":110,"amount":0.3,"unit":"g","g_l":0.3,"mmol_l":2.11208,"optional":"no"},{"recipe No.":8,"solution":"Mineral solution ","solution_id":3048,"amount":5,"unit":"ml","optional":"no"},{"recipe No.":9,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":16,"compound":"Sodium acetate trihydrate","compound_id":1954,"amount":2,"unit":"mM","g_l":0.27216,"mmol_l":2,"optional":"no"},{"recipe No.":17,"compound":"Sodium thiosulfate pentahydrate","compound_id":1092,"amount":2,"unit":"mM","g_l":0.496372,"mmol_l":2,"optional":"no"},{"recipe No.":18,"compound":"Sodium bicarbonate","compound_id":2149,"amount":1,"unit":"mM","mmol_l":1,"optional":"no"}],"steps":[{"recipe No.":11,"step":"Adjust pH to 7.2."},{"recipe No.":12,"step":"Prepare the medium under a nitrogen atmosphere. Dispense 5 ml into 25 ml tubes fitted with rubber stoppers."},{"recipe No.":13,"step":"Autoclave at 121 \u00b0C for 15 min."},{"recipe No.":15,"step":"after autoklaving, add the following from anaerobic, sterile (autoclaved) stock solutions (e.g., 100x)"},{"step":"Before inoculation 2% sterile O2 or 10% sterile air is added to the headspace."}],"equipment":{"5233":"Autoclave"},"main solution":"yes"},{"id":3048,"name":"Mineral solution ","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na2-EDTA","compound_id":34,"amount":3.7,"unit":"g","g_l":3.7,"mmol_l":11.0052,"optional":"no"},{"recipe No.":2,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":1.1,"unit":"g","g_l":1.1,"mmol_l":3.95662,"optional":"no"},{"recipe No.":3,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.0288,"unit":"g","g_l":0.0288,"mmol_l":0.100153,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":0.0984,"unit":"g","g_l":0.0984,"mmol_l":0.497208,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":0.031,"unit":"g","g_l":0.031,"mmol_l":0.501294,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":0.0238,"unit":"g","g_l":0.0238,"mmol_l":0.183304,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":0.0172,"unit":"g","g_l":0.0172,"mmol_l":0.10089,"optional":"no"},{"recipe No.":8,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.0242,"unit":"g","g_l":0.0242,"mmol_l":0.100021,"optional":"no"},{"recipe No.":9,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 6.0 with KOH"}],"equipment":{"165":"Autoclave","428":"Anaerobic workbench"},"main solution":"no"}]}