{"medium":{"id":1455,"name":"ALTERNATIVE RUMEN BACTERIA MEDIUM","complex_medium":"yes","min_pH":6.6,"max_pH":6.8,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1455.pdf"},"solutions":[{"id":2989,"name":"Main sol. 1455","volume":1006,"recipe":[{"recipe No.":1,"compound":"K2HPO4","compound_id":10,"amount":0.23,"unit":"g","g_l":0.228628,"mmol_l":1.31263,"optional":"no"},{"recipe No.":2,"compound":"KH2PO4","compound_id":11,"amount":0.23,"unit":"g","g_l":0.228628,"mmol_l":1.68004,"optional":"no"},{"recipe No.":3,"compound":"NaCl","compound_id":43,"amount":0.45,"unit":"g","g_l":0.447316,"mmol_l":7.65428,"optional":"no"},{"recipe No.":4,"compound":"(NH4)2SO4","compound_id":27,"amount":0.45,"unit":"g","g_l":0.447316,"mmol_l":3.38517,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.06,"unit":"g","g_l":0.0596421,"mmol_l":0.405687,"optional":"no"},{"recipe No.":6,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.09,"unit":"g","g_l":0.0894632,"mmol_l":0.362969,"optional":"no"},{"recipe No.":7,"compound":"Brain heart infusion","compound_id":186,"attribute":"BD BBL","amount":6,"unit":"g","g_l":5.96421,"optional":"no"},{"recipe No.":8,"compound":"Yeast extract","compound_id":16,"amount":5,"unit":"g","g_l":4.97018,"optional":"no"},{"recipe No.":9,"solution":"Volatile fatty acid mixture","solution_id":608,"amount":3.1,"unit":"ml","optional":"no"},{"recipe No.":10,"solution":"Haemin solution","solution_id":124,"attribute":"0.05% w/v","amount":2,"unit":"ml","optional":"no"},{"recipe No.":11,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000497018,"mmol_l":0.00197881,"optional":"no"},{"recipe No.":12,"compound":"NaHCO3","compound_id":55,"amount":6.4,"unit":"g","g_l":6.36183,"mmol_l":75.7298,"optional":"no"},{"recipe No.":13,"compound":"Disodium succinate","compound_id":1189,"amount":5,"unit":"g","g_l":4.97018,"mmol_l":30.6707,"optional":"no"},{"recipe No.":14,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":15,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.3,"unit":"g","g_l":0.298211,"mmol_l":1.69789,"optional":"no"},{"recipe No.":16,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":0.298211,"mmol_l":1.2416,"optional":"no"},{"recipe No.":17,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except bicarbonate, succinate, vitamins, cysteine and sulfide, then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the bicarbonate and equilibrate the medium with the CO2 gas to reach a pH between 6.5 and 7.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add succinate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Prior to use adjust pH of complete medium to 6.6 - 6.8, if necessary."}],"equipment":{"364":"Filter","2387":"Autoclave","3178":"Hungate tubes","4175":"Gassing station"},"main solution":"yes"},{"id":608,"name":"Volatile fatty acid mixture","volume":1000,"recipe":[{"recipe No.":1,"compound":"Acetic acid","compound_id":380,"amount":548.5,"unit":"ml","g_l":573.182,"mmol_l":9545.09,"optional":"no"},{"recipe No.":2,"compound":"Propionic acid","compound_id":381,"amount":193.5,"unit":"ml","g_l":192.145,"mmol_l":2593.76,"optional":"no"},{"recipe No.":3,"compound":"Butyric acid","compound_id":314,"amount":129,"unit":"ml","optional":"no"},{"recipe No.":4,"compound":"n-Valeric acid","compound_id":288,"amount":32.25,"unit":"ml","g_l":30.2827,"mmol_l":296.512,"optional":"no"},{"recipe No.":5,"compound":"iso-Butyric acid","compound_id":377,"amount":32.25,"unit":"ml","g_l":30.6375,"mmol_l":347.719,"optional":"no"},{"recipe No.":6,"compound":"DL-2-Methylbutyric acid","compound_id":223,"amount":32.25,"unit":"ml","optional":"no"},{"recipe No.":7,"compound":"iso-Valeric acid","compound_id":378,"amount":32.25,"unit":"ml","g_l":30.0247,"mmol_l":293.986,"optional":"no"}],"Sol. No.":21,"main solution":"no"},{"id":124,"name":"Haemin solution","volume":100,"recipe":[{"recipe No.":1,"compound":"Haemin","compound_id":149,"amount":50,"unit":"mg","g_l":0.5,"mmol_l":0.766989,"optional":"no"},{"recipe No.":2,"compound":"NaOH","compound_id":256,"attribute":"1 N","amount":1,"unit":"ml","optional":"no"},{"recipe No.":3,"compound":"Distilled water","compound_id":4,"amount":100,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated."}],"equipment":{"1875":"Filter"},"Sol. No.":9,"main solution":"no"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}