{"medium":{"id":"141a","name":"METHANOCALDOCOCCUS LAUENSIS MEDIUM","complex_medium":"no","min_pH":6.2,"max_pH":6.2,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141a.pdf"},"solutions":[{"id":243,"name":"Main sol. 141a","volume":1013,"recipe":[{"recipe No.":1,"compound":"KCl","compound_id":61,"amount":0.34,"unit":"g","g_l":0.335637,"mmol_l":4.50217,"optional":"no"},{"recipe No.":2,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":4,"unit":"g","g_l":3.94867,"mmol_l":19.4226,"optional":"no"},{"recipe No.":3,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":3.45,"unit":"g","g_l":3.40573,"mmol_l":13.8177,"optional":"no"},{"recipe No.":4,"compound":"NH4Cl","compound_id":42,"amount":0.25,"unit":"g","g_l":0.246792,"mmol_l":4.61379,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.14,"unit":"g","g_l":0.138203,"mmol_l":0.940063,"optional":"no"},{"recipe No.":6,"compound":"K2HPO4","compound_id":10,"amount":0.14,"unit":"g","g_l":0.138203,"mmol_l":0.79347,"optional":"no"},{"recipe No.":7,"compound":"NaCl","compound_id":43,"amount":18,"unit":"g","g_l":17.769,"mmol_l":304.055,"optional":"no"},{"recipe No.":8,"solution":"Modified Wolin's mineral solution","solution_id":241,"amount":10,"unit":"ml","optional":"no"},{"recipe No.":9,"compound":"Fe(NH4)2(SO4)2 x 6 H2O","compound_id":247,"attribute":"0.1% w/v","amount":2,"unit":"ml","g_l":0.00197433,"optional":"no"},{"recipe No.":10,"compound":"Na-acetate","compound_id":23,"amount":1,"unit":"g","g_l":0.987167,"mmol_l":12.0337,"optional":"no"},{"recipe No.":11,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000493583,"mmol_l":0.00196514,"optional":"no"},{"recipe No.":12,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":13,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.5,"unit":"g","g_l":0.493583,"mmol_l":2.81026,"optional":"no"},{"recipe No.":14,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.5,"unit":"g","g_l":0.493583,"mmol_l":2.05504,"optional":"no"},{"recipe No.":15,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients (except vitamins, cysteine, and sulfide), sparge medium  with  80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust the pH of the final medium to 6.2."},{"step":"For incubation use sterile 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure."}],"equipment":{"581":"Filter","1358":"Autoclave","3405":"Hungate tubes","4006":"Gassing station"},"main solution":"yes"},{"id":241,"name":"Modified Wolin's mineral solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.84765,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":3,"unit":"g","g_l":3,"mmol_l":12.1716,"optional":"no"},{"recipe No.":3,"compound":"MnSO4 x H2O","compound_id":14,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.9583,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","g_l":1,"mmol_l":17.1116,"optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.359693,"optional":"no"},{"recipe No.":6,"compound":"CoSO4 x 7 H2O","compound_id":198,"amount":0.18,"unit":"g","g_l":0.18,"mmol_l":1.16132,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.680203,"optional":"no"},{"recipe No.":8,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.18,"unit":"g","g_l":0.18,"mmol_l":0.625954,"optional":"no"},{"recipe No.":9,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0400503,"optional":"no"},{"recipe No.":10,"compound":"AlK(SO4)2 x 12 H2O","compound_id":908,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.0421596,"optional":"no"},{"recipe No.":11,"compound":"H3BO3","compound_id":37,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.161708,"optional":"no"},{"recipe No.":12,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0413309,"optional":"no"},{"recipe No.":13,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.231483,"optional":"no"},{"recipe No.":14,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":0.3,"unit":"mg","g_l":0.0003,"mmol_l":0.00114062,"optional":"no"},{"recipe No.":15,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":0.4,"unit":"mg","g_l":0.0004,"mmol_l":0.00121267,"optional":"no"},{"recipe No.":16,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH."}],"Sol. No.":17,"main solution":"no"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}