{"medium":{"id":"135c","name":"NEOMOORELLA THERMOACETICA MEDIUM","complex_medium":"yes","min_pH":6.5,"max_pH":6.5,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135c.pdf"},"solutions":[{"id":234,"name":"Main sol. 135c","volume":1021,"recipe":[{"recipe No.":1,"compound":"NH4Cl","compound_id":42,"amount":1,"unit":"g","g_l":0.979432,"mmol_l":18.3106,"optional":"no"},{"recipe No.":2,"compound":"KH2PO4","compound_id":11,"amount":0.33,"unit":"g","g_l":0.323213,"mmol_l":2.37508,"optional":"no"},{"recipe No.":3,"compound":"K2HPO4","compound_id":10,"amount":0.45,"unit":"g","g_l":0.440744,"mmol_l":2.53045,"optional":"no"},{"recipe No.":4,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.1,"unit":"g","g_l":0.0979432,"mmol_l":0.397374,"optional":"no"},{"recipe No.":5,"solution":"Modified Wolin's mineral solution","solution_id":241,"amount":20,"unit":"ml","optional":"no"},{"recipe No.":6,"compound":"Yeast extract","compound_id":16,"amount":2,"unit":"g","g_l":1.95886,"optional":"no"},{"recipe No.":7,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000489716,"mmol_l":0.00194974,"optional":"no"},{"recipe No.":8,"compound":"NaHCO3","compound_id":55,"amount":1,"unit":"g","g_l":0.979432,"mmol_l":11.6589,"optional":"no"},{"recipe No.":9,"compound":"D-Fructose","compound_id":33,"amount":10,"unit":"g","g_l":9.79432,"mmol_l":54.3646,"optional":"yes"},{"recipe No.":10,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":11,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.5,"unit":"g","g_l":0.489716,"mmol_l":2.78824,"optional":"no"},{"recipe No.":12,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.5,"unit":"g","g_l":0.489716,"mmol_l":2.03894,"optional":"no"},{"recipe No.":13,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients except bicarbonate, fructose, vitamins, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be at 6.5."},{"step":"Note: For autotrophic growth fructose is omitted and a gas atmosphere of 80% H2 and 20% CO2 is used."}],"equipment":{"448":"Autoclave","1271":"Hungate tubes","3999":"Gassing station"},"main solution":"yes"},{"id":241,"name":"Modified Wolin's mineral solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"Nitrilotriacetic acid","compound_id":189,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.84765,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":3,"unit":"g","g_l":3,"mmol_l":12.1716,"optional":"no"},{"recipe No.":3,"compound":"MnSO4 x H2O","compound_id":14,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.9583,"optional":"no"},{"recipe No.":4,"compound":"NaCl","compound_id":43,"amount":1,"unit":"g","g_l":1,"mmol_l":17.1116,"optional":"no"},{"recipe No.":5,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.359693,"optional":"no"},{"recipe No.":6,"compound":"CoSO4 x 7 H2O","compound_id":198,"amount":0.18,"unit":"g","g_l":0.18,"mmol_l":1.16132,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.1,"unit":"g","g_l":0.1,"mmol_l":0.680203,"optional":"no"},{"recipe No.":8,"compound":"ZnSO4 x 7 H2O","compound_id":35,"amount":0.18,"unit":"g","g_l":0.18,"mmol_l":0.625954,"optional":"no"},{"recipe No.":9,"compound":"CuSO4 x 5 H2O","compound_id":121,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0400503,"optional":"no"},{"recipe No.":10,"compound":"AlK(SO4)2 x 12 H2O","compound_id":908,"amount":0.02,"unit":"g","g_l":0.02,"mmol_l":0.0421596,"optional":"no"},{"recipe No.":11,"compound":"H3BO3","compound_id":37,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.161708,"optional":"no"},{"recipe No.":12,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":0.01,"unit":"g","g_l":0.01,"mmol_l":0.0413309,"optional":"no"},{"recipe No.":13,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":0.03,"unit":"g","g_l":0.03,"mmol_l":0.231483,"optional":"no"},{"recipe No.":14,"compound":"Na2SeO3 x 5 H2O","compound_id":107,"amount":0.3,"unit":"mg","g_l":0.0003,"mmol_l":0.00114062,"optional":"no"},{"recipe No.":15,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":0.4,"unit":"mg","g_l":0.0004,"mmol_l":0.00121267,"optional":"no"},{"recipe No.":16,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH."}],"Sol. 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