{"medium":{"id":"120a","name":"METHANOSARCINA BARKERI MEDIUM","complex_medium":"yes","min_pH":6.5,"max_pH":6.8,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120a.pdf"},"solutions":[{"id":215,"name":"Main sol. 120a","volume":1024,"recipe":[{"recipe No.":1,"compound":"K2HPO4","compound_id":10,"amount":0.35,"unit":"g","g_l":0.341797,"mmol_l":1.96236,"optional":"no"},{"recipe No.":2,"compound":"KH2PO4","compound_id":11,"amount":0.23,"unit":"g","g_l":0.224609,"mmol_l":1.65051,"optional":"no"},{"recipe No.":3,"compound":"NH4Cl","compound_id":42,"amount":0.5,"unit":"g","g_l":0.488281,"mmol_l":9.12846,"optional":"no"},{"recipe No.":4,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.5,"unit":"g","g_l":0.488281,"mmol_l":1.98105,"optional":"no"},{"recipe No.":5,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.25,"unit":"g","g_l":0.244141,"mmol_l":1.66065,"optional":"no"},{"recipe No.":6,"compound":"NaCl","compound_id":43,"amount":2.25,"unit":"g","g_l":2.19727,"mmol_l":37.5987,"optional":"no"},{"recipe No.":7,"solution":"FeSO4 x 7 H2O solution (0.1% w/v)","solution_id":5861,"amount":2,"unit":"ml","optional":"no"},{"recipe No.":8,"solution":"Trace element solution SL-10","solution_id":595,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":9,"compound":"Yeast extract","compound_id":16,"attribute":"OXOID","amount":2,"unit":"g","g_l":1.95312,"optional":"no"},{"recipe No.":10,"compound":"Casitone","compound_id":84,"attribute":"BD BBL","amount":2,"unit":"g","g_l":1.95312,"optional":"no"},{"recipe No.":11,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.000488281,"mmol_l":0.00194403,"optional":"no"},{"recipe No.":12,"compound":"NaHCO3","compound_id":55,"amount":0.85,"unit":"g","g_l":0.830078,"mmol_l":9.88106,"optional":"no"},{"recipe No.":13,"compound":"Methanol","compound_id":226,"attribute":"50% v/v","amount":20,"unit":"ml","g_l":15.4688,"mmol_l":482.765,"optional":"no"},{"recipe No.":14,"solution":"Wolin's vitamin solution (10x)","solution_id":5980,"amount":1,"unit":"ml","optional":"no"},{"recipe No.":15,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.3,"unit":"g","g_l":0.292969,"mmol_l":1.66805,"optional":"no"},{"recipe No.":16,"compound":"Na2S x 9 H2O","compound_id":54,"amount":0.3,"unit":"g","g_l":0.292969,"mmol_l":1.21978,"optional":"no"},{"recipe No.":17,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.5 and dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Methanol (50% v/v stock solution) and the reducing agents are each autoclaved separately under 100% N2 gas atmosphere as concentrated solutions in tightly closed tubes. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Appropriate volumes of the solutions are injected into the sterile medium with hypodermic syringes. Adjust pH of the complete medium to 6.5 - 6.8, if necessary."}],"equipment":{"106":"Autoclave","2217":"Hungate tubes","3228":"Filter","3992":"Gassing station"},"main solution":"yes"},{"id":5861,"name":"FeSO4 x 7 H2O solution (0.1% w/v)","volume":1000,"recipe":[{"recipe No.":2,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":1,"unit":"g","g_l":1,"mmol_l":3.59693,"optional":"no"},{"recipe No.":3,"compound":"H2SO4","compound_id":122,"attribute":"0.1 N","amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"The ferrous sulfate solution is not stable and should be freshly prepared."}],"main solution":"no"},{"id":595,"name":"Trace element solution SL-10","volume":1000,"recipe":[{"recipe No.":1,"compound":"HCl","compound_id":68,"attribute":"25%","amount":10,"unit":"ml","g_l":2.5,"mmol_l":68.5683,"optional":"no"},{"recipe No.":2,"compound":"FeCl2 x 4 H2O","compound_id":260,"amount":1.5,"unit":"g","g_l":1.5,"mmol_l":7.54482,"optional":"no"},{"recipe No.":3,"compound":"ZnCl2","compound_id":64,"amount":70,"unit":"mg","g_l":0.07,"mmol_l":0.513573,"optional":"no"},{"recipe No.":4,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":5,"compound":"H3BO3","compound_id":37,"amount":6,"unit":"mg","g_l":0.006,"mmol_l":0.0970246,"optional":"no"},{"recipe No.":6,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":190,"unit":"mg","g_l":0.19,"mmol_l":1.46335,"optional":"no"},{"recipe No.":7,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":2,"unit":"mg","g_l":0.002,"mmol_l":0.0117314,"optional":"no"},{"recipe No.":8,"compound":"NiCl2 x 6 H2O","compound_id":40,"amount":24,"unit":"mg","g_l":0.024,"mmol_l":0.185187,"optional":"no"},{"recipe No.":9,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":36,"unit":"mg","g_l":0.036,"mmol_l":0.148791,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":990,"unit":"ml","optional":"no"}],"steps":[{"step":"First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml."}],"Sol. No.":2,"main solution":"no"},{"id":5980,"name":"Wolin's vitamin solution (10x)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Biotin","compound_id":46,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0818632,"optional":"no"},{"recipe No.":2,"compound":"Folic acid","compound_id":138,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.0453104,"optional":"no"},{"recipe No.":3,"compound":"Pyridoxine hydrochloride","compound_id":519,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.486287,"optional":"no"},{"recipe No.":4,"compound":"Thiamine HCl","compound_id":1207,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.148236,"optional":"no"},{"recipe No.":5,"compound":"Riboflavin","compound_id":136,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.132837,"optional":"no"},{"recipe No.":6,"compound":"Nicotinic acid","compound_id":139,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.406141,"optional":"no"},{"recipe No.":7,"compound":"Calcium D-(+)-pantothenate","compound_id":1512,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.209846,"optional":"no"},{"recipe No.":8,"compound":"Vitamin B12","compound_id":18,"amount":1,"unit":"mg","g_l":0.001,"mmol_l":0.00073779,"optional":"no"},{"recipe No.":9,"compound":"p-Aminobenzoic acid","compound_id":47,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.364591,"optional":"no"},{"recipe No.":10,"compound":"(DL)-alpha-Lipoic acid","compound_id":252,"amount":50,"unit":"mg","g_l":0.05,"mmol_l":0.24233,"optional":"no"},{"recipe No.":11,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"}]}