{"medium":{"id":1145,"name":"MS-MEDIUM (MODIFIED)","complex_medium":"no","source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1145.pdf"},"solutions":[{"id":2296,"name":"Main sol. 1145","volume":1000,"recipe":[{"recipe No.":1,"compound":"Double distilled water","compound_id":1021,"attribute":"anaerobic","amount":800,"unit":"ml","optional":"no"},{"recipe No.":2,"solution":"MS Buffer","solution_id":2297,"amount":200,"unit":"ml","optional":"no"},{"recipe No.":3,"compound":"Elemental sulphur","compound_id":884,"amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":4,"compound":"NaS2O3 x 5 H2O","compound_id":1018,"amount":2,"unit":"g","g_l":2,"optional":"no"},{"recipe No.":5,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":7,"unit":"g","g_l":7,"mmol_l":28.4003,"optional":"no"},{"recipe No.":6,"compound":"KCl","compound_id":61,"amount":0.48,"unit":"g","g_l":0.48,"mmol_l":6.43863,"optional":"no"},{"recipe No.":7,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.4,"unit":"g","g_l":0.4,"mmol_l":2.72081,"optional":"no"},{"recipe No.":8,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":0.8,"unit":"g","g_l":0.8,"mmol_l":3.93501,"optional":"no"},{"recipe No.":9,"solution":"Solution A","solution_id":2298,"amount":20,"unit":"ml","optional":"no"},{"recipe No.":10,"solution":"Solution B","solution_id":2299,"amount":1.5,"unit":"ml","optional":"no"},{"recipe No.":11,"solution":"Solution C - Trace mineral solution (Ferguson and Mah, 1983)","solution_id":2300,"amount":10,"unit":"ml","optional":"no"}],"steps":[{"step":"Each of the solutions (A, B, and C) is prepared with anaerobic double distilled water."},{"step":"The medium is prepared anaerobically under nitrogen gas and dispensed into Hungate tubes or serum bottle sealed with rubber septa. The medium to gas phase ratio should be about 1:10 (v:v). \r\nSterilise the medium by heating at 100\u00b0 C for 3 hours on three consecutive days (e.g., in a water bath)."},{"step":"Add  4% O2 for microaerophilic conditions."}],"equipment":{"3002":"Hungate tubes","3722":"Anaerobic workbench","5076":"Water bath"},"main solution":"yes"},{"id":2297,"name":"MS Buffer","volume":200,"recipe":[{"recipe No.":1,"compound":"NaOH","compound_id":256,"amount":100,"unit":"mM","g_l":3.9997,"mmol_l":100,"optional":"no"}],"steps":[{"step":"100 mM NaOH saturated with CO2"}],"equipment":{"2157":"Anaerobic workbench"},"main solution":"no"},{"id":2298,"name":"Solution A","volume":1000,"recipe":[{"recipe No.":1,"compound":"NH4Cl","compound_id":42,"amount":100,"unit":"g","g_l":100,"mmol_l":1869.51,"optional":"no"},{"recipe No.":2,"compound":"MgCl2 x 6 H2O","compound_id":79,"amount":100,"unit":"g","g_l":100,"mmol_l":491.877,"optional":"no"},{"recipe No.":3,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":40,"unit":"g","g_l":40,"mmol_l":272.081,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust to pH 4 with HCl"}],"main solution":"no"},{"id":2299,"name":"Solution B","volume":1000,"recipe":[{"recipe No.":1,"compound":"K2HPO4 x 3 H2O","compound_id":244,"amount":200,"unit":"g","g_l":200,"mmol_l":1148.26,"optional":"no"},{"recipe No.":2,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"main solution":"no"},{"id":2300,"name":"Solution C - Trace mineral solution (Ferguson and Mah, 1983)","volume":1000,"recipe":[{"recipe No.":1,"compound":"Na2-EDTA x 2 H2O","compound_id":676,"amount":500,"unit":"mg","g_l":0.5,"mmol_l":1.48718,"optional":"no"},{"recipe No.":2,"compound":"Na2WO4 x 2 H2O","compound_id":249,"amount":30,"unit":"mg","g_l":0.03,"mmol_l":0.0909504,"optional":"no"},{"recipe No.":3,"compound":"CoCl2 x 6 H2O","compound_id":38,"amount":150,"unit":"mg","g_l":0.15,"mmol_l":1.15528,"optional":"no"},{"recipe No.":4,"compound":"CuCl2 x 2 H2O","compound_id":39,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.117314,"optional":"no"},{"recipe No.":5,"compound":"MnCl2 x 4 H2O","compound_id":36,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.505293,"optional":"no"},{"recipe No.":6,"compound":"NiSO4 x 6 H2O","compound_id":1030,"amount":20,"unit":"mg","g_l":0.02,"mmol_l":0.129236,"optional":"no"},{"recipe No.":7,"compound":"FeSO4 x 7 H2O","compound_id":12,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.359693,"optional":"no"},{"recipe No.":8,"compound":"H2SeO3","compound_id":894,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.0775374,"optional":"no"},{"recipe No.":9,"compound":"ZnCl2","compound_id":64,"amount":100,"unit":"mg","g_l":0.1,"mmol_l":0.733676,"optional":"no"},{"recipe No.":10,"compound":"H3BO3","compound_id":37,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.161708,"optional":"no"},{"recipe No.":11,"compound":"AlCl3 x 6 H2O","compound_id":677,"amount":40,"unit":"mg","g_l":0.04,"mmol_l":0.165679,"optional":"no"},{"recipe No.":12,"compound":"Na2MoO4 x 2 H2O","compound_id":9,"amount":10,"unit":"mg","g_l":0.01,"mmol_l":0.0413309,"optional":"no"},{"recipe No.":13,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"steps":[{"step":"Adjust pH to 3.0 with HCl"}],"main solution":"no"}]}