{"medium":{"id":"104d","name":"MALEDIVIBACTER MEDIUM","complex_medium":"yes","min_pH":7.3,"max_pH":7.5,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104d.pdf"},"solutions":[{"id":180,"name":"Main sol. 104d","volume":1000,"recipe":[{"recipe No.":1,"compound":"Trypticase peptone","compound_id":124,"attribute":"BD BBL","amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":2,"compound":"Meat peptone","compound_id":646,"attribute":"pepsin-digested","amount":5,"unit":"g","g_l":5,"optional":"no"},{"recipe No.":3,"compound":"Yeast extract","compound_id":16,"amount":10,"unit":"g","g_l":10,"optional":"no"},{"recipe No.":4,"solution":"Salt solution","solution_id":174,"amount":40,"unit":"ml","optional":"no"},{"recipe No.":5,"compound":"NaCl","compound_id":43,"amount":60,"unit":"g","g_l":60,"mmol_l":1026.69,"optional":"no"},{"recipe No.":6,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":5,"unit":"g","g_l":5,"mmol_l":20.286,"optional":"no"},{"recipe No.":7,"compound":"Sodium resazurin","compound_id":1852,"attribute":"0.1% w/v","amount":0.5,"unit":"ml","g_l":0.0005,"mmol_l":0.00199068,"optional":"no"},{"recipe No.":8,"compound":"Betaine x H2O","compound_id":212,"amount":6,"unit":"g","g_l":6,"mmol_l":51.2181,"optional":"no"},{"recipe No.":9,"compound":"L-Cysteine HCl x H2O","compound_id":770,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.8468,"optional":"no"},{"recipe No.":10,"compound":"Distilled water","compound_id":4,"amount":960,"unit":"ml","optional":"no"}],"steps":[{"step":"Dissolve ingredients (except betaine and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Adjust the pH to 7.2. Thereafter, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add betaine and cysteine from sterile, anoxic stock solutions prepared under 100% N2. Adjust the pH of the complete medium to 7.3 - 7.5."}],"equipment":{"1584":"Autoclave","2373":"Hungate tubes","3976":"Gassing station"},"main solution":"yes"},{"id":174,"name":"Salt solution","volume":1000,"recipe":[{"recipe No.":1,"compound":"CaCl2 x 2 H2O","compound_id":7,"amount":0.25,"unit":"g","g_l":0.25,"mmol_l":1.70051,"optional":"no"},{"recipe No.":2,"compound":"MgSO4 x 7 H2O","compound_id":8,"amount":0.5,"unit":"g","g_l":0.5,"mmol_l":2.02859,"optional":"no"},{"recipe No.":3,"compound":"K2HPO4","compound_id":10,"amount":1,"unit":"g","g_l":1,"mmol_l":5.74132,"optional":"no"},{"recipe No.":4,"compound":"KH2PO4","compound_id":11,"amount":1,"unit":"g","g_l":1,"mmol_l":7.34835,"optional":"no"},{"recipe No.":5,"compound":"NaHCO3","compound_id":55,"amount":10,"unit":"g","g_l":10,"mmol_l":119.038,"optional":"no"},{"recipe No.":6,"compound":"NaCl","compound_id":43,"amount":2,"unit":"g","g_l":2,"mmol_l":34.2231,"optional":"no"},{"recipe No.":7,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"}],"Sol. No.":14,"main solution":"no"}]}